Our Methodology
Methodology for O2P™ Determination of Peroxide Value & Fatty Acids Content
At NPRI, we employ rigorous methodologies to ensure the quality and stability of our O2P™ (Oil-to-Powder) products. This process involves determining the peroxide value and fatty acid content using advanced techniques. Our meticulous approach ensures that our nutraceuticals maintain their nutritional integrity and efficacy.
Peroxide Value Determination
Our O2P™ product undergoes a modified method to determine its peroxide value, which is crucial for assessing lipid oxidation. Here is an overview of our process:
- Acid Hydrolysis: The oil powder is subjected to acid hydrolysis to eliminate interference from matrix ingredients. This step is essential for accurate peroxide value determination.
- Oil Extraction: After hydrolysis, the oil is extracted using ether/chloroform while replacing any air with nitrogen (N2) to prevent oxidation.
- Solvent Evaporation: The solvent is evaporated, and the recovered oil is prepared for peroxide value determination.
- Peroxide Value Determination: We use the USP REDOX METHOD or the A.O.C.S. OFFICIAL METHOD CD-8-53 / JA 8-87 to measure the peroxide value, ensuring the reliability of our results.
Fatty Acid Content Determination
To determine the fatty acid content in our O2P™ product, we use a GLC (Gas-Liquid Chromatography) method. Here are the steps involved:
- Solvent Recovery and Esterification: After extracting the oil via solvent recovery, we esterify the fatty acids to form fatty acid methyl esters.
- GLC Analysis: The methyl esters are injected into a GLC system with a capillary column to separate the isomer components.
- Quantitation: The response obtained from the GLC system is compared to a standard containing known fatty acids. The calculation is based on the individual peak area of each isomer divided by the total peak area, resulting in the percentage of fatty acids present.
Important Note
Before determining the peroxide value of O2P™ (Oil-to-Powder), the resultant powder undergoes acid hydrolysis. The sample is digested in a 25% HCL solution under low heat with a reflux condenser and a flow of nitrogen (N2). The ether solvent is then evaporated, and the oil is recovered for peroxide value determination based on the A.O.C.S. method.
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